ea0059p130 | Neuroendocrinology and pituitary | SFEBES2018
Alobaid Hussah
, Voliotis Margaritis
, Tsaneva-Atanasova Krasimira
, McArdle Craig
Gonadotropin-releasing hormone (GnRH) is a hypothalamic neuropeptide that acts via GnRHR on the pituitary gonadotrope. It is secreted in pulses and acts via GnRHR to activate ERK and Nuclear Factor of Activated T-cells (NFAT), mediating GnRH effects on gonadotropin expression. We monitor their activation by high content imaging (fluorescence staining for ppERK and nuclear translocation of an NFAT1c-EFP reporter) in fixed LbT2 gonadotroph cells. Single cell measures reveal high...